Chondroitinase B cleaves dermatan sulfate (chondroitin sulfate B).
Synonyms：Chondroitin B eliminase
Gene Source: Native Flavobacterium heparinum
EC Number: 188.8.131.52
CAS Number: 52227-83-5
MDL Number: MFCD00166444
Catalyzed Reaction: Chondroitinase B cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin B).The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.
Substrate Specificity: Dermatan sulfate (chondroitin sulfate B).
Molecular weight: 55 kDa
Isoelectric point: 9.4 – 9.6
pH optimum for activity: 7 – 8
pH range for activity: 5 – 10
Optimal temperature range: 20 ℃ – 37 ℃
Purity: ≥ 90% by reversed phase HPLC analysis.
Specific Activity: ≥200 IU/mg (substrate: dermatan sulfate). One international unit (IU) of chondroitinase B is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 ℃ and pH 8.0.
Stability: 12 months frozen at – 20 ℃ in aqueous buffers containing sodium phosphate and sucrose 5%.
Chondroitinase B is useful in many applications, for example:
Catalog number: AG00-8058
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