Chondroitinase B

Chondroitinase B cleaves dermatan sulfate (chondroitin sulfate B).

Chondroitinase B

Usage

  1. Research reagent (glycobiology, preparation of oligosaccharide libraries from dermatan sulfate).
  2. Determination of contents of chondrotin sulfates by HPLC.

General Information

Synonyms:Chondroitin B eliminase

Gene Source: Native Flavobacterium heparinum

EC Number: 4.2.2.19

CAS Number: 52227-83-5

MDL Number: MFCD00166444

Catalyzed Reaction: Chondroitinase B cleaves, via an elimination mechanism, polysaccharide chains containing 1-4 linkages between hexosamines and iduronic acid residues in dermatan sulfate (chondroitin B).The reaction yields oligosaccharide products (mainly disaccharides) containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm.

Substrate Specificity: Dermatan sulfate (chondroitin sulfate B).

Properties:

Molecular weight: 55 kDa

Isoelectric point: 9.4 – 9.6

pH optimum for activity: 7 – 8

pH range for activity: 5 – 10

Optimal temperature range: 20 ℃ – 37 ℃

Purity: ≥ 90% by reversed phase HPLC analysis.

Specific Activity: ≥200 IU/mg (substrate: dermatan sulfate). One international unit (IU) of chondroitinase B is defined as the amount of enzyme that will liberate 1.0 μmole unsaturated oligosaccharides from dermatan sulfate per minute at 30 ℃ and pH 8.0.

Stability: 12 months frozen at – 20 ℃ in aqueous buffers containing sodium phosphate and sucrose 5%.

Applications

Chondroitinase B is useful in many applications, for example:

  1. As research reagent (glycosaminoglycan degradation).
  2. For the preparation of di- and oligo-saccharides of dermatan sulfate.

References:

  1. A comparative Study Between a Chondroitinase B and a Chondroitinase AC from Flavobacterium heparinum. M.Y.M. Michelacci and D.C.P. Dietrich, in Biochemical Journal (1975) 151: 121-129.

Order Information

Catalog number: AG00-8058

Package size & price:

Packing size Price (USD)
5 IU inquiry
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