Published on 2015/2/27 11:51:50
Chondroitin sulfate is used in dietary supplements or osteoarthritis treatment. It is a mixture of sulfated glycosaminoglycan (GAG) chains of repeated disaccharide building blocks. Each disaccharide building block is composed of a N-acetylgalactosamine residue and a glucuronic acid residue.
Due to the structural complexity of chondroitin sulfate, most quantitative methods to determine its contents are not satisfactory. However, by applying chondroitinase ABC or AC to completely digest chondroitin sulfate into unsatisfied disaccharides, followed by separation by a HPLC system equipped with a SAX column and a UV detector with its wavelength set to 232 nm, a quantitative method can be easily established.
Either chondroitinase ABC or chondroitinase AC is suitable for chondroitin analysis. Please refer to the procedure provided by your local regulatory agencies or some international standards organizations (e.g., AOAC).