We offer the full range of products for heparin analysis, such as structural elucidation, oligosaccharide sequencing and activity assays.
By using mixture of heparinase I, heparinase III and heparinase III, enoxaparin sodium can be depolymerized and the level of 1,6-hydro forms can be determined.
Oligosaccharides are prepared from marine creatures such as shrimps, crabs, algin and red algae by chemical depolymerization, then purified by chromatography.
Aglyco offers six series of oligosaccharides:
Aglyco's chromogenic substrates are designed to be acted by coagulation factors and have been used in diagnostic kits for antithrombin tests. For production of diagnostic kits, we offer prepacks up to 10 grams.
Heparinase I and heparinase II can be used as additives in blood tests.
Multifunctional plasma mutagenesis systems (MPMS) enable automated mutagenesis by providing plasma, ultraviolet and chemical agents in a single device.
MPMS can be used in a wide range of applications, such as production strain improvement, metabolic network investigation and mutation pattern identification.
By using MPMS and high-throughout screening (HTS) in combination, users are able to perform microbial breeding very conveniently.
Global production of pharmaceutical heparin (Hp) is increasing, and the production process from raw mucosal material results in large amounts of waste by-products. These contain lower sulfated Hp-like and heparan sulfate (HS), as well as other glycosaminoglycans, which are bioactive entities with pharmaceutical potential. Here we describe the first purification, structural and functional characterisation of Hp-like and HS polysaccharides from the four major by-product fractions of standard heparin production. Analysis of the by-products by disaccharide composition analysis and NMR demonstrated a range of structural characteristics which differentiate them from Hp (particularly reduced sulfation and sulfated disaccharide content), and that they are each distinct. Functional properties of the purified by-products varied, each displaying distinct anticoagulant profiles in different assays, and all exhibiting significantly lower global and specific inhibition of the coagulation pathway than Hp. The by-products retained the ability to promote cell proliferation via fibroblast growth factor receptor signalling, with only minor differences between them. These collective analyses indicate that they represent an untapped and economical source of structurally-diverse Hp-like and HS polysaccharides with the potential for enhancing future structure-activity studies and uncovering new biomedical applications of these important natural products.Read